Detection of C. trachomatis in the Serum of the Patients with Urogenital Chlamydiosis
Figure 3
C. trachomatis-specific amplicons recovered in RT-PCR reactions with RNA isolated from serum isolates of the patients with urogenital chlamydiosis. RNA was isolated from infected McCoy and RT-PCR reaction performed as described in Section 2. A percentage of 1.2% agarose gel contains the following lanes: 1: size marker; 2 and 3: amplification products obtained in PCR with RNA isolated from serum of patient no. 1 and patient no. 2; 4: amplification product using RNA from reference strain L2/434/Bu; 5: RNA extraction control; 6: positive control; 7: negative control.
