Functional and Molecular Characterization of Ex Vivo Cultured Epiretinal Membrane Cells from Human Proliferative Diabetic Retinopathy
Figure 2
Angiogenic factors secreted by the fvERM cells. The fvERM cells were treated with 100 ng/mL TNFα for 24 hours. Supernatants were collected, and cytokine levels were determined by a Human Angiogenesis Array Proteome Profiler. Map of the 55 angiogenesis-related proteins detected on the membranes (a). Panel of the secreted proteins of control and TNFα treated cells (b). Flow cytometric surface marker analysis of the TNFα treated cells ((c), (d)). (Data shown are mean ± SD of 3 independent experiments on 3 different donor fvERM samples).
