Functional and Molecular Characterization of Ex Vivo Cultured Epiretinal Membrane Cells from Human Proliferative Diabetic Retinopathy
Figure 3
Intracellular propagation of calcium signal upon mechanical stimulation in fvERM cells from PDR. Mechanical stimulation by a glass pipette (a). Image showing the positions of the regions of interest (ROIs) presented in different colors to which correspond the traces on (b), (c), and (d). The colored traces show the intracellular calcium concentration changes in time before and after the mechanical stimulation, with different colors corresponding to the regions of the cell marked with corresponding colors (c). Enlarged region of the traces in (c) showing that calcium increase starts in the cell region marked in blue and then propagates with a time delay to the cell regions marked in red-green and then yellow (d). Color-coded images showing the intracellular calcium concentration distribution for four selected instants marked in (c) with asterisk (e).
