Identification of Protein Complex Associated with LYT1 of Trypanosoma cruzi

Figure 1

Diagrams representing the pTREXn-EGFP and pTREXn-LYT1s+n-EGFP constructs, transcripts, and protein products (not to scale). The presence and relative position of trans-splicing acceptor sites (SAS₁ and SAS₂), miniexon (ME), translations initiation sites (ATG1 and ATG₂), signal sequence (SS), nuclear localization sequence (NLS), and cleavage site (CS) are shown for the mRNA (EGFP mRNA, LYT1s-EGFP mRNA, and LYT1n-EGFP mRNA) and corresponding proteins (EGFP, LYT1s-EGFP, and LYT1-EGFP). Striped boxes represent EGFP sequences. Arrowheads pointing to filled boxes represent LYT1 sequences. Restriction sites are given. Details of the plasmid construction are described in “Section 2.”