493525.fig.002a
(a)
493525.fig.002b
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493525.fig.002c
(c)
Figure 2: Analysis of EGFP and LYT1s+n-EGFP chimeric proteins expression. (a) Protein extracts of WT parasites and EGFP and LYT1s+n-EGFP transgenic parasites were run on polyacrylamide gels under denaturing conditions and transferred, and the exogenous proteins were revealed using an α-EGFP antibody. (b) Protein extracts of WT parasites and EGFP and LYT1s+n-EGFP transgenic parasites were dotted under native conditions and revealed using an α-EGFP or α-T. cruzi antibody. (c) Protein extract of WT parasites analyzed by SDS-PAGE and Coomassie-blue stain.