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BioMed Research International
Volume 2013 (2013), Article ID 496586, 12 pages
http://dx.doi.org/10.1155/2013/496586
Research Article

Production, Characterization and Antioxidant Potential of Protease from Streptomyces sp. MAB18 Using Poultry Wastes

1Marine Biotechnology Laboratory, Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea
2Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu 608 502, India
3Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea

Received 29 April 2013; Revised 20 June 2013; Accepted 8 July 2013

Academic Editor: Periasamy Anbu

Copyright © 2013 Panchanathan Manivasagan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: Optimization of Significant Variables Using Response Surface Methodology (RSM): The statistical optimization of protease production using RSM. (A) peptone and Na2CO3; (B) NaCl and Na2CO3; (C) WCF and peptone; (D) peptone and NaCl; (E) WCF and Na2CO3; (F) WCF and NaCl.

Table S1: Screening of Parameters Using the Plackett-Burman Design: Plackett-Burman experimental design matrix with protease production levels. Statistical analysis of the model. Statistical parameters for selected the linear polynomial model using Plackett-Burman design.

Table S2: Optimization of Significant Variables Using Response Surface Methodology (RSM): Central composite factor experimental design along with experimental and predicted values. Analysis for variance of protease production.

Table S3 A: Optimization of Protease Production: Effect of various carbon and nitrogen sources on production of protease from Streptomyces sp. MAB18.

Table S3 B: Purification of Protease: Summary of purification steps of protease from Streptomyces sp. MAB18.

Table S4: Effect of Metal Ions and Chemicals on Enzyme Activity: Effect of metal ions and chemicals on activity of the purified protease from Streptomyces sp. MAB18.

  1. Supplementary Materials