ASPic-GeneID: A Lightweight Pipeline for Gene Prediction and Alternative Isoforms Detection

Figure 1

Graphical overview of ASPic-GeneID. In the absence of ASPic introns, the dynamic programming algorithm implemented in GeneID (called Genamic) assembles the most likely gene structure according to frame-compatible exons with the highest combined score. When ASPic introns are provided, they act as anchors in the chaining of exons so that exons with intron-compatible splice sites are always joined together if they conform to a valid gene model. In this hypothetical example, the “First” exon with the highest score (in orange) is replaced by the one with the lowest score (in blue), but which possesses an ASPic intron-compatible splice site (in red).