BioMed Research International / 2013 / Article / Tab 5 / Research Article
Effect of C/N Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra- and Extracellular Inulinase and Invertase from Aspergillus niger ATCC 20611 Table 5 Composition of various experiments of the central composite design (CCD) for independent variables and predicted and experimental values of responses.
Run no. Sucrose (w/v) Yeast extract (w/v) NaNO3 (w/v) Zn+2 (v/v) Triton X-100 (v/v) Extracellular inulinase (U/mL) Extracellular invertase (U/mL) Biomass mg/mL C/N Actual Predicted Actual Predicted Actual Predicted Actual Predicted 1 16.00 4.00 1.00 2.00 0.50 1032.23 1140.22 1318.67 1311.74 24.25 24.51 2.1 2.09 2 16.00 1.00 3.00 2.00 0.50 1430.86 1367.56 1764.30 1698.08 36.29 37.83 1.5 1.56 3 4.00 4.00 3.00 1.00 1.50 219.53 123.44 458.12 461.95 19.00 16.24 2.6 2.65 4 16.00 4.00 3.00 1.00 0.50 862.46 867.01 903.05 833.45 21.18 19.06 2.5 2.51 5 16.00 4.00 1.00 1.00 1.50 884.57 748.36 1128.66 1059.05 20.21 20.19 2.4 2.45 6 16.00 1.00 1.00 2.00 1.50 1332.80 1297.23 1395.53 1416.14 34.61 34.69 1.7 1.63 7 4.00 1.00 3.00 2.00 1.50 1235.41 1172.11 1342.99 1293.56 29.04 30.75 2.0 2.02 8 4.00 4.00 1.00 2.00 1.50 1030.99 1038.33 1293.56 1376.84 23.20 23.00 2.2 2.19 9 16.00 1.00 3.00 1.00 1.50 1402.18 1366.60 1621.43 1625.26 35.40 34.74 1.6 1.61 10 4.00 4.00 3.00 2.00 0.50 930.16 970.30 1202.20 1198.66 22.44 21.60 2.3 2.38 11 4.00 1.00 1.00 1.00 0.50 1259.39 1163.32 1377.56 1321.19 29.76 29.64 1.9 1.96 12 4.00 2.50 2.00 1.50 1.00 1282.62 1490.61 1382.73 1404.97 30.81 33.02 1.8 1.60 13 16.00 2.50 2.00 1.50 1.00 1598.38 1756.51 2068.23 2256.15 37.11 38.01 1.4 1.35 14 10.00 1.00 2.00 1.50 1.00 2167.92 2461.75 2413.59 2561.17 39.37 36.80 1.1 1.02 15 10.00 4.00 2.00 1.50 1.00 1904.32 1976.61 2352.84 2415.42 38.69 44.36 1.2 1.03 16 10.00 2.50 1.00 1.50 1.00 2247.08 2399.61 2452.98 2481.99 39.62 39.62 1.0 0.98 17 10.00 2.50 4.00 1.50 1.00 1881.45 2095.04 2306.64 2487.78 38.07 41.18 1.3 1.07 18 10.00 2.50 2.00 1.00 1.00 2259.90 2619.30 2496.12 2684.04 40.96 46.63 0.9 0.73 19 10.00 2.50 2.00 2.00 1.00 3681.21 3687.93 3854.48 3876.72 73.39 70.82 0.1 0.02 20 10.00 2.50 2.00 1.50 0.50 2287.32 2294.04 2528.36 2731.03 43.17 44.44 0.8 0.60 21 10.00 2.50 2.00 1.50 1.50 2383.92 2743.32 2677.46 2684.94 43.29 45.13 0.7 0.64 22 10.00 2.50 2.00 1.50 1.00 2726.29 2681.88 2854.61 2887.25 49.57 48.29 0.6 0.61 23 10.00 2.50 2.00 1.50 1.00 2959.09 2681.88 3079.77 2887.25 48.69 48.29 0.4 0.61 24 10.00 2.50 2.00 1.50 1.00 3166.54 2681.88 3105.58 2887.25 52.16 48.29 0.3 0.61 25 10.00 2.50 2.00 1.50 1.00 2807.25 2681.88 2939.38 2887.25 49.92 48.29 0.5 0.61 26 10.00 2.50 2.00 1.50 1.00 3214.72 2681.88 3297.57 2887.25 53.55 48.29 0.2 0.61