Effect of C/N Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra- and Extracellular Inulinase and Invertase from Aspergillus niger ATCC 20611

Table 6

Optimum conditions for independent variables and predicted and experimental values of responses.

Run no.	Sucrose (w/v)	Yeast extract (w/v)	NaNO3 (w/v)	Zn^+2 (v/v)	Triton X-100 (v/v)	Extracellular inulinase (U/mL)		Extracellular invertase (U/mL)		Biomass mg/mL		C/N
						Actual	Predicted	Actual	Predicted	Actual	Predicted	Actual	Predicted
2	7.17	1.47	1.28	2.00	0.50	3802.43	3733.14	3858.98	3862.30	72.54	73.49	0.02	0.00
3	7.35	1.73	1.09	1.99	0.50	3754.98	3812.80	3930.42	3917.98	72.98	73.39	0.15	0.10
4	7.13	1.76	1.42	2.00	0.50	3793.65	3765.34	3906.97	3885.80	73.03	73.48	0.06	0.08
5	8.95	2.16	1.00	2.00	0.50	3698.96	3768.61	3865.38	3866.43	74.07	73.47	0.02	0.03