Ovarian and Breast Cancer Spheres Are Similar in Transcriptomic Features and Sensitive to Fenretinide
Figure 5
Fenretinide inhibited the formation of sphere cells in ovarian and breast cancer cells. (a) Data summary and analysis of cell viability in A2780 cells. A2780 cells were treated with different concentrations of fenretinide for 48 hrs. Cell viability was determined by the MTT assay. Means and standard errors of three independent experiments were shown. (b) Illustration showed the A2780, MCF7 and SUM159 sphere cells. A2780, MCF7, and SUM159 cells were seeded in sphere-forming condition with (down) and without (up) 3 μM fenretinide, the spheres were photographed using inverted microscope (magnification 10x). The results were obtained from three independent experiments. (c) Representative dot-plots illustrating apoptotic status in A2780 parental and sphere cells. A2780 parental and sphere cells were treated with 3 μM fenretinide for 48 hrs. The apoptotic cells were detected through Annexin V/PI assay. (d) Data summary and analysis of apoptotic index in A2780 parental and sphere cells. Means and standard errors of apoptosis percentages of A2780 parental and sphere cells treated with fenretinide from three experiments were shown. ***, statistically significant difference between A2780 parental and sphere cells treated with fenretinide ().
