Dose- and Time-Dependent Apoptosis Induced by Avian H9N2 Influenza Virus in Human Cells

Table 1

Viral and cellular genes expression levels in response to H9N2 influenza virus of A549, HeLa, and HepG2 cells evaluated by quantitative RT-PCR at the indicated time point. Ct values were determined for viral NP, Mx, and 𝛽-actin genes.

	Mx		𝛽-actin		NP
	MOI 0.1	MOI 2.0	MOI 0.1	MOI 2.0	MOI 0.1	MOI 2.0
A549
16 hpi	19.46	18.15	17.82	17.76	20.85	20.90
24 hpi	24.93	20.27	17.96	17.93	25.72	25.75
36 hpi	22.76	20.03	18.07	18.01	28.84	28.53
48 hpi	21.89	19.68	18.15	18.22	28.28	28.11
HeLa
16 hpi	16.72	16.56	17.74	17.72	19.82	19.77
24 hpi	20.53	20.82	17.83	17.76	21.90	21.80
36 hpi	18.86	18.94	18.21	18.24	23.57	23.64
48 hpi	19.02	18.17	18.20	18.17	24.03	23.92
HepG2
16 hpi	—*	—	17.85	17.91	21.02	20.94
24 hpi	—	—	17.74	17.86	25.64	25.37
36 hpi	—	—	18.65	18.57	28.43	28.68
48 hpi	—	—	18.42	18.44	28.12	28.44

∗Undetectable or very low expression level.