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Figure 5: (a–e) Immunohistochemical staining of TGF-β1 in kidney of normal group (a), PBS group (b), UTMD group (c), MSCs group (d), and UTMD + MSCs group (e). The brown area was the positive expression of TGF-β1 and TGF-β1 expression significantly decreased after MSCs transplantation combined with UTMD technique. versus groups. (f) represents the histogram of integrated optical density (IOD) of TGF-β1 expression analyzed using Image-Pro plus 6.0 software. (g) IL-10 levels in renal cortices were determined by ELISA and they were obviously higher in UTMD + MSCs group than those in PBS group, UTMD group, and MSCs group. versus groups. (h and i) represents Western blot (h) and summary of densitometric analyses (i) for synaptopodin expression, which showed that synaptopodin expression increased after MSCs infusion and there was a greater improvement in UTMD + MSCs group. versus groups.