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BioMed Research International
Volume 2013 (2013), Article ID 526837, 7 pages
http://dx.doi.org/10.1155/2013/526837
Research Article

CYP3A5*3 and C3435T MDR1 Polymorphisms in Prognostication of Drug-Resistant Epilepsy in Children and Adolescents

1Department of Neuropediatrics, Medical University of Silesia, 16 Medykow Street, 40-752 Katowice, Poland
2Department of Human Anatomy, Medical University of Silesia, 18 Medykow Street, 40-752 Katowice, Poland
3Department of Biochemistry and Medical Genetics, School of Health Care, Medical University of Silesia, 18 Medykow Street, 40-752 Katowice, Poland
4Department of General and Molecular Biology and Genetics, Medical University of Silesia, 18 Medykow Street, 40-752 Katowice, Poland
5CoE Research and Teaching of Molecular Biology of Matrix and Nanotechnology, Network of CoE BioMedTech Silesia, 40-752 Katowice, Poland

Received 30 April 2013; Accepted 25 June 2013

Academic Editor: Luca Cucullo

Copyright © 2013 Ewa Emich-Widera et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Drug-resistant epilepsies still remain one of the most profound problems of contemporary epileptology. Several mechanisms of drug resistance are possible; among them, genetic factors have a prominent place. Much importance is attached to genes, which encode enzymes that metabolize antiepileptic drugs CYP 3A, which belong to the family of cytochromes P450 and the genome of multidrug resistance, such as multidrug resistance 1 (MDR1) that expresses P-glycoprotein (P-gp), a drug transporter protein. The aim of the study was to assess the relation between polymorphism of gene CYP3A5 and polymorphism C3435T of MDR1 gene with the occurrence of focal, drug-resistant epilepsy in children and youths up to 18 years of age. The study comprised 85 patients, and their age range was from 33 months to 18 years of age, suffering from epilepsy, partly responding well to treatment, partly drug resistant. The polymorphism of both genes has been analysed using the PCR-RFLP method. The study failed to corroborate association between polymorphism CYP3A5*3 and C3435T polymorphism in MDR1 gene and pharmacoresistant epilepsy. The results of our research do not confirm the prognostic value of the polymorphisms examined in the prognostication of drug resistance in epilepsies.