Biodistribution and Molecular Studies on Orally Administered Nanoparticle-AON Complexes Encapsulated with Alginate Aiming at Inducing Dystrophin Rescue in mdx Mice
Figure 6
Immunoblotting of dystrophin in the intestine of untreated mdx mice (NT), ZM2-AON treated mice, and alginate-coated ZM2-AON treated mice. Intestine from WT mice were used as positive controls. For WT samples, the total protein loaded was 1/10 (15 μg) of the quantity used in the other lanes (150 μg). Quantitation performed by densitometric analysis of autoradiographic bands followed by normalization with the quantity of total protein loaded on the gels showed % () of dystrophin recovery in the intestine from alginate ZM2-AON-treated mice compared to WT mice. The arrows show the bands of dystrophin (427 kD).
