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BioMed Research International
Volume 2013 (2013), Article ID 529589, 18 pages
http://dx.doi.org/10.1155/2013/529589
Research Article

Recovery of Fertility in Azoospermia Rats after Injection of Adipose-Tissue-Derived Mesenchymal Stem Cells: The Sperm Generation

1Ankalife IVF and Women Health Centre, Ankara, Turkey
2Stem Cell Department, Institute of Health Sciences, Kocaeli University Center for Stem Cell and Gene Therapies Research and Practice, Izmit, 41380 Kocaeli, Turkey
3Urology Department, Faculty of Medicine, Ufuk University, Ankara, Turkey
4Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey
5Pathology Department, Ankara Hospital, Ankara, Turkey

Received 10 October 2012; Revised 6 December 2012; Accepted 9 December 2012

Academic Editor: Thomas Skutella

Copyright © 2013 Cihangir Cakici et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The recent reports on the treatment of azoospermia patients, in which spermatozoa could not be traced in their testes, are focused more on the potential use of adult stem cells, like mesenchymal stem cells (MSCs). The aim of this study was to demonstrate the potential use of MSCs derived from adipose tissue in the treatment of azoospermia using rat disease models. After busulfan application, the rats ( ) were injected with the GFP+ MSCs into left rete testes. After 12 weeks, the testes with cell injection (right testes) were compared to control (left testes) after dimensional and immunohistochemical analyses. Testes treated with MSCs appeared morphologically normal, but they were atrophic in rats without stem cell treatment, in which the seminiferous tubules were empty. Spermatogenesis was detected, not in every but in some tubules of cell-treated testes. GFP+/VASA+ and GFP+/SCP1+ cells in testes indicated the transdifferentiation of MSCs into spermatogenetic cells in the appropriate microenvironment. Rats with cell treatment were mated to show the full recovery of spermatogenesis, and continuous generations were obtained. The expression of GFP was detected in the mesenchymal stem cells derived from adipose tissue and bone marrow and also in the sperms of offspring. In conclusion, MSCs might be studied for the same purpose in humans in future.