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Figure 3: Microscopic images of rAT-MSCs differentiated into adipocytes (a, b), osteoblasts (d), neuron-glial like cells (e), and endothelial cells (f). The arrows indicate the neutral lipid vacuoles stained with Oil Red O (b). rAT-MSCs were undifferentiated in standard culture medium (c). Phase contrast microscope images of the rAT-MSCs differentiated into osteogenic lineage, where calcified nodules (arrow) were stained with Alizarin red S (d). Differentiation of rAT-MSCs to neuron-glial like cells after 3 days (e). Endothelial tube formation by rAT-MSCs on Matrigel (f). Immunostaining of cells for GFAP (green) and beta-tubulin (red) differentiated cells (g1–g3). Increased cytoplasmic and nuclear staining of differentiated cells for c-Fos (red) and NF (green; cytoplasmic and membranous) was observed (h1–h3).