Recovery of Fertility in Azoospermia Rats after Injection of Adipose-Tissue-Derived Mesenchymal Stem Cells: The Sperm Generation

Table 1

Immunocytochemical properties of rAT-MSCs.

Antibody/marker	Dilution	Source	Detection
CD 26	1 : 50	Santa Cruz Bio.	+
CD 34 (C-18)	1 : 150	Santa Cruz Bio.	∅
CD 45 (H-230)	1 : 150	Santa Cruz Bio.	∅
CD 71 (K-20)	1 : 150	Santa Cruz Bio.	∅
CD105/Endoglin (M-20)	1 : 100	Santa Cruz Bio.	+
c-Fos (4)	1 : 50	Santa Cruz Bio.	+
Collagen II (2B1.5)	Predilute	Thermo Scientific	+
Collagen Ia1 (D-13)	1 : 50	Santa Cruz Bio.	+
𝛽-tubulin	1 : 50	Santa Cruz Bio.	+
Nestin (Rat-401)	1 : 50	Santa Cruz Bio.	+
Vimentin (C-20)	1 : 100	Santa Cruz Bio.	+
Fibronectin (EP5)	1 : 100	Santa Cruz Bio.	+
ASMA	1 : 800	Thermo Scientific	+
Myogenin (F5D)	Predilute	Thermo Scientific	+
MAP 2a, b (AP20)	Predilute	Thermo Scientific	+
GFAP	Predilute	Thermo Scientific	+
Osteocalcin (FL-100)	1 : 50	Santa Cruz Bio.	+
Osteonectin (SPARC)	1 : 50	Millipore	+
Osteopontin (AKm2A1)	1 : 50	Santa Cruz Bio.	+
Ki67	1 : 300	Thermo Scientific	+
PCNA	1 : 200	Thermo Scientific	+
Tenascin-C	1 : 50	Santa Cruz Bio.	+
Cytokeratin 18	1 : 50	Santa Cruz Bio.	∅

+: positive; ∅: lack of marker expression.