Table 1: Immunocytochemical properties of rAT-MSCs.

Antibody/markerDilutionSourceDetection

CD 26 1 : 50 Santa Cruz Bio. +
CD 34 (C-18) 1 : 150 Santa Cruz Bio.
CD 45 (H-230) 1 : 150 Santa Cruz Bio.
CD 71 (K-20) 1 : 150 Santa Cruz Bio.
CD105/Endoglin (M-20) 1 : 100 Santa Cruz Bio. +
c-Fos (4) 1 : 50 Santa Cruz Bio. +
Collagen II (2B1.5) Predilute Thermo Scientific +
Collagen Ia1 (D-13) 1 : 50 Santa Cruz Bio. +
-tubulin 1 : 50 Santa Cruz Bio. +
Nestin (Rat-401) 1 : 50 Santa Cruz Bio. +
Vimentin (C-20) 1 : 100 Santa Cruz Bio. +
Fibronectin (EP5) 1 : 100 Santa Cruz Bio. +
ASMA 1 : 800 Thermo Scientific +
Myogenin (F5D) Predilute Thermo Scientific +
MAP 2a, b (AP20) Predilute Thermo Scientific +
GFAP Predilute Thermo Scientific +
Osteocalcin (FL-100) 1 : 50 Santa Cruz Bio. +
Osteonectin (SPARC) 1 : 50 Millipore +
Osteopontin (AKm2A1) 1 : 50 Santa Cruz Bio. +
Ki67 1 : 300 Thermo Scientific +
PCNA 1 : 200 Thermo Scientific +
Tenascin-C 1 : 50 Santa Cruz Bio. +
Cytokeratin 18 1 : 50 Santa Cruz Bio.

+: positive; : lack of marker expression.