Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo

Figure 1

Overexpression of GDNF in vitro and in vivo. (a) Representative images of immunofluorescent staining for 𝛼-actinin (AA) and glial cell line derived neurotrophic factor (GDNF) in the control and GDNF cardiomyocytes after culture for 4 days. (b) Quantitative analysis of GDNF positive cells area in the control and GDNF cardiomyocytes (^∗𝑃<0.05, versus control, 𝑛=4). (c) Representative images of immunofluorescent staining for 𝛼-actinin (AA) and GDNF in the control and GDNF grafts at 4 weeks after transplantation. (d) Quantitative analysis of GDNF positive cells area in the control and GDNF grafts (^∗𝑃<0.05, versus control, 𝑛=4). Scale bars indicate 100 μm. AA: 𝛼-actinin; GDNF: glial cell line derived neurotrophic factor.

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