Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo

Figure 4

Sympathetic innervation in engrafted engineered cardiomyocyte sheets. (a) Representative images of immunofluorescent staining for 𝛼-actinin (AA) and tyrosine hydroxylase (TH) in the control and GDNF grafts at 1, 2, and 4 weeks after cardiomyocyte sheets transplantation. Arrows indicate sympathetic nerves in the grafts. (b) Quantitative analysis of TH positive nerve area in the control and GDNF cardiomyocyte sheet grafts (^∗𝑃<0.05, versus control, 𝑛=5). Scale bars indicate 200 μm. AA: 𝛼-actinin; TH: tyrosine hydroxylase.  

(a)

(b)