Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo

Figure 5

Patterns of growing nerves (GAP43), sympathetic nerves (TH), parasympathetic nerves (ChAT), and autonomic nerves (NF-M) in the grafts. (a)–(d), (e)–(h), and (i)–(l) show representative images of triple immunostaining for NF-M/GAP43/GFP, NF-M/ChAT/GFP, and NF-M/TH/GFP in the GDNF grafts at 4 weeks after transplantation. Scale bar indicates 200 μm. GAP43: growth associated protein 43; TH: tyrosine hydroxylase; ChAT: choline acetyltransferase; NF-M: neurofilament-M.