Angiotensin II AT ₁ Receptors Are Involved in Neuronal Activation Induced by Amphetamine in a Two-Injection Protocol

Figure 2

Average number of Fos-IR neurons in brain nuclei: IL cortex (a), VTA (c), in response to a challenge of saline (𝑛 = 4-5) or amphetamine (𝑛 = 5–8), 21 days after a pretreatment with cv or veh and a treatment with amph or sal (veh-sal, cv-sal, veh-amph, and cv-amph). Values are means ± SEM. ^∗𝑃<0.05 significantly different from the other groups in response to the same challenge (3-way ANOVA, post hoc Bonferroni). Average number of Fos/TH-IR neurons in VTA (b), in response to a challenge of saline (𝑛 = 4-5) or amphetamine (𝑛 = 5–8), 21 days after a pretreatment with cv or veh and a treatment with amph or sal (veh-sal, cv-sal, veh-amph and cv-amph). Values are means ± SEM. ^∗𝑃<0.05 significantly different from the salin-challenged groups (3-way ANOVA, post hoc Bonferroni).

(a) Infralimbic cortex

(b) Ventral tegmental area-TH (+) neurons

(c) Ventral tegmental area-total neurons