BioMed Research International

Review Article

Improved Succinate Production by Metabolic Engineering

Coproduction of succinate and other high-value-added products using different bacteria species.


Strains	Strategies	Products	Substrates	Reference

E. coli KJ071	Deletion of LDH, ADHE, FOCA-PFLB, MGSA, PTA-ACKA.	33.1 g L⁻¹ succinate, 69.2 g L⁻¹ malate	Glucose	[8]
E. coli KJ073	Deletion of LDH, ADHE, FOCA-PFLB, MGSA, POXB, PTA-ACKA.	78.9 g L⁻¹ succinate, 15.8 g L⁻¹ malate	Glucose	[8]
E. coli SBS990MG	Deletion of LDH, ADHE, PTA-ACKA. Overexpression of PYC and AAT	1.22 g L⁻¹ isoamyl acetate, 5.37 g L⁻¹ succinate	Glucose and isoamyl alcohol	[9]
E. coli QZ1112	Deletion of SDHA, POXB, PTA-ACKA. Overexpression of PHBCAB	24.6 g L⁻¹ succinate, 4.95 g L⁻¹ polyhydroxybutyrate	Glucose	[10]
E. coli KNSP1	Deletion of FADR, PTAS, ATOC, FADA, SDHA, PTA-ACKA. Overexpression of PHAC1	21.07 g L⁻¹ succinate, 0.54 g L⁻¹ 3-hydroxyoctanoate + 3-hydroxydecanoate	Glycerol and fatty acid	[49]
K. pneumoniae LDH526	Deletion of LDH	102.1 g L⁻¹ 1,3-propanediol, 13.8 g L⁻¹succinate	Glycerol	[50]
K. pneumoniae CICC 10011	Enhanced CO₂ level in the medium	77.1 g L⁻¹ 2,3-butanediol, 28.7 g L⁻¹succinate	Glucose	[51]
A. succinogenes ATCC 55618	Fractional treatment and separate utilization for succinate production.	64 g L⁻¹succinate, glucoamylase	Wheat	[52]