A Phytase Characterized by Relatively High pH Tolerance and Thermostability from the Shiitake Mushroom Lentinus edodes

Table 3

Substrate specificity of L. edodes phytase.

Substrate	Relative activity (%)
Sodiumm phytate	100.0 ± 5.1
β-glycerophosphate	98.5 ± 3.7
ADP	103.4 ± 6.2
G-6-P	127.9 ± 3.9
AMP	143.8 ± 7.0
F-6-P	158.2 ± 4.6
ATP	208.1 ± 6.4

The phytase activity towards sodium phytate (5.0 mM) was regarded as 100%. Phytase activity was assayed with increasing concentration of Pi using the standard phytase assay. Results are presented as mean ± SD (𝑛 = 3).