Table 3: Substrate specificity of L. edodes phytase.

SubstrateRelative activity (%)

Sodiumm phytate100.0 ± 5.1
β-glycerophosphate98.5 ± 3.7
ADP103.4 ± 6.2
G-6-P127.9 ± 3.9
AMP143.8 ± 7.0
F-6-P158.2 ± 4.6
ATP208.1 ± 6.4

The phytase activity towards sodium phytate (5.0 mM) was regarded as 100%. Phytase activity was assayed with increasing concentration of Pi using the standard phytase assay. Results are presented as mean ± SD ( = 3).