A Phytase Characterized by Relatively High pH Tolerance and Thermostability from the Shiitake Mushroom Lentinus edodes

Table 4

Effects of metal ions and EDTA on phytase activity.

	Relative activity (%)
	1 mM	5 mM
K^+	101.5 ± 3.2	104.9 ± 2.8
Ca^2+	107.6 ± 3.1	114.8 ± 5.3
Mg^2+	102.7 ± 0.8	103.5 ±3.5
Mn^2+	95.4 ± 5.1	69.6 ± 2.7
Zn^2+	90.2 ± 2.1	76.5 ± 1.6
Cu^2+	104.6 ± 2.5	82.8 ± 2.3
Fe^3+	96.9 ± 3.0	100.2 ± 1.7
Al^3+	67.6 ± 1.3	32.5 ± 0.9
EDTA	100.7 ± 2.8	103.4 ± 2.3

The phytase activity in the absence of metal ions was regarded as 100%. Data are given as means ± SD, 𝑛 = 3.