Characterization and Dynamic Behavior of Wild Yeast during Spontaneous Wine Fermentation in Steel Tanks and Amphorae

Table 3

Sizes of digested and undigested nested PCR products representing different yeast species derived using the T-RFLP method.

Yeast species	Size (bp)
	Nested PCR	Digested
Aureobasidium pullulans *	254	—
Botryotinia fuckeliana *	—	223
Bulleromyces albus	195	164
Candida glabrata	478	224
Candida zemplinina	200
Candida zeylanoides	269	238
Cryptococcus flavus	169	134
Cryptococcus magnus	211	—
Dekkera bruxellensis	168	134
Epicoccum nigrum *	216	—
Filobasidium floriforme	237	204
Hanseniaspora guilliermondii	364	66
Hanseniaspora uvarum	365	66
Hyphopichia burtonii	159	124
Issatchenkia orientalis	178	148
Kluyveromyces lactis	305	273
Metschnikowia pulcherrima	140	107
Metschnikowia sp.	348	—
Pichia angusta	379	351
Pichia anomala	258	228
Pichia fermentans	156	122
Pichia holstii	—	248
Pichia kluyveri	162	128
Pichia membranifaciens	165	130
Rhodotorula mucilaginosa	229	198
Saccharomyces bayanus	435	405
Saccharomyces cerevisiae	441	410
Saccharomyces pastorianus	435	405
Sporidiobolus pararoseus	222	197
Torulaspora delbrueckii (wild yeast isolate)	370	340
Williopsis saturnus	252	134
Zygosaccharomyces florentinus	244	213

*Molds.