Development of a Generic Microfluidic Device for Simultaneous Detection of Antibodies and Nucleic Acids in Oral Fluids
Figure 4
The consecutive flow protocol. (a) Schematic for the analysis of the antibody path, the Test Line was a proprietary peptide mix (OraSure Technologies), and the Flow Line anti-human IgG. (b) Schematic showing consecutive flow applied to detect RT-PCR amplicons provided with a digoxigenin and biotin hapten as described by Corstjens et al. [8], which was developed to detect antibodies against infectious disease pathogens. For analysis of amplicons, the Test line was antidigoxigenin and the Flow Line digoxigenin.
