Validation Thin Layer Chromatography for the Determination of Acetaminophen in Tablets and Comparison with a Pharmacopeial Method
Table 4
Acetaminophen assay [mg · tablet−1] obtained from ten repeated different analyses by TLC-densitometric and UV-spectrodensitometric methods for tablets of three different pharmaceutical manufactures.
No.
Product 1
Product 2
Product 3
Assay by TLC-densitometric method
Assay by UV-spectrophotometric method
Assay by TLC-densitometric method
Assay by UV-spectrophotometric method
Assay by TLC-densitometric method
Assay by UV-spectrophotometric method
1
500.3
502.3
509.0
504.5
502.4
505.7
2
503.5
491.3
488.1
497.5
496.8
489.8
3
488.3
486.5
499.6
494.7
509.6
511.2
4
495.2
494.4
504.4
498.6
497.8
492.3
5
500.5
493.5
494.3
503.6
502.7
508.9
6
491.8
490.6
499.6
489.9
505.8
506.7
7
489.1
494.5
499.2
497.3
497.6
491.2
8
504.2
506.2
501.5
501.9
509.8
505.9
9
503.1
506.9
496.9
492.9
506.7
497.9
10
491.2
490.8
498.8
493.9
508.9
504.6
Average assay
496.7
495.7
499.1
497.5
503.8
501.4
Label claimed
500.0
500.0
500.0
500.0
500.0
500.0
Standard deviation (SD)
6.4
7.0
5.6
4.8
5.1
7.9
Coefficient of variation CV, %
1.27
1.40
1.12
0.96
1.01
1.58
TLC-densitometric method compared with UV-spectrophotometric method