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BioMed Research International
Volume 2013 (2013), Article ID 545983, 8 pages
http://dx.doi.org/10.1155/2013/545983
Research Article

Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors

1Departamento de Cirugía General y Digestiva (Seccion B), Hospital Universitario “12 de Octubre”, Universidad Complutense, Avenida de Córdoba, s/n, 28041 Madrid, Spain
2Departamento de Fisiología, Universidad de Valencia, Avenida Blasco Ibáñez 15, 46010 Valencia, Spain
3INCLIVA, Instituto Investigación Sanitaria, Hospital Clínico Universitario, Avenida Blasco Ibáñez 15, 46010 Valencia, Spain
4Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Arzobispo Morcillo 2, 28029 Madrid, Spain

Received 22 April 2013; Revised 7 August 2013; Accepted 2 October 2013

Academic Editor: Gary E. Gallick

Copyright © 2013 Eduardo Ferrero et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to analyze whether tyrosine phosphorylation in tumoral arteries may modulate their vascular response. To do this, mesenteric arteries supplying blood flow to colorectal tumors or to normal intestine were obtained during surgery and prepared for isometric tension recording in an organ bath. Increasing tyrosine phosphorylation with the phosphatase inhibitor, sodium orthovanadate produced arterial contraction which was lower in tumoral than in control arteries, whereas it reduced the contraction to noradrenaline in tumoral but not in control arteries and reduced the relaxation to bradykinin in control but not in tumoral arteries. Protein expression of VEGF-A and of the VEGF receptor FLT1 was similar in control and tumoral arteries, but expression of the VEGF receptor KDR was increased in tumoral compared with control arteries. This suggests that tyrosine phosphorylation may produce inhibition of the contraction in tumoral mesenteric arteries, which may increase blood flow to the tumor when tyrosine phosphorylation is increased by stimulation of VEGF receptors.