Figure 4: Leptin effect on AANAT activity and melatonin synthesis in cultured rat pineal gland. The glands were challenged with leptin (Lep 1 nM) alone and in association with norepinephrine (NE 1 μM). (a) AANAT activity was determined using cultured pineal glands, values are expressed in pmols/gland/h. (b) Melatonin content was quantified from pineal gland culture medium, values are expressed as ng/well. One-way ANOVA, followed by Bonferroni’s post hoc test. versus control, versus NE 1 μM. glands/group and each experiment was repeated 3 times.