Development of an Ammonium Sulfate DNA Extraction Method for Obtaining Amplifiable DNA in a Small Number of Cells and Its Application to Clinical Specimens

<table>Comparison of <i><i>β</i>-globin </i>gene amplification using DNA extracted from various numbers of SNU 790 cells and PTC cells on archival smear slides. We compared PCR products amplified from DNA extracted using three different methods to determine the efficacy of DNA extraction. This assay used <i><i>β</i>-globin</i> primers that amplify a 110 bp fragment of genomic DNA. (a) Ammonium sulfate DNA extraction method; (b) Pinpoint Slide DNA Isolation System; (c) TaKaRa DEXPAT; PTC: papillary thyroid carcinoma.</table>

BioMed Research International

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Figure 1

Figure 1: Development of an Ammonium Sulfate DNA Extraction Method for Obtaining Amplifiable DNA in a Small Number of Cells and Its Application to Clinical Specimens 

Figure 1 | Development of an Ammonium Sulfate DNA Extraction Method for Obtaining Amplifiable DNA in a Small Number of Cells and Its Application to Clinical Specimens 