Development of an Ammonium Sulfate DNA Extraction Method for Obtaining Amplifiable DNA in a Small Number of Cells and Its Application to Clinical Specimens
Table 2
Comparison of DNA yield and purity extracted using three different methods from various numbers of PTC cells on archival smear slide.
No. of cells
Extraction method
DNA concentration
(ng/μL)
Total DNA yield (ng)
Purity A260/A280
P value*
25 ()
A
0.013 ± 0.002
0.652 ± 0.081
1.6 ± 0.1
P = 0.001
B
0.001 ± 0.001
0.070 ± 0.041
1.0 ± 0.0
C
0.004 ± 0.002
0.190 ± 0.122
1.4 ± 0.2
50 ()
A
0.059 ± 0.007
2.975 ± 0.337
1.7 ± 0.2
P = 0.000
B
0.007 ± 0.003
0.327 ± 0.175
1.2 ± 0.1
C
0.017 ± 0.006
0.834 ± 0.277
1.3 ± 0.2
100 ()
A
0.104 ± 0.008
5.184 ± 0.388
1.5 ± 0.2
P = 0.001
B
0.056 ± 0.007
2.777 ± 0.356
1.6 ± 0.2
C
0.085 ± 0.006
4.241 ± 0.291
1.6 ± 0.3
200 ()
A
0.179 ± 0.008
8.947 ± 0.413
1.6 ± 0.2
P = 0.000
B
0.075 ± 0.011
3.745 ± 0.550
1.8 ± 0.3
C
0.136 ± 0.016
6.797 ± 0.812
1.4 ± 0.2
500 ()
A
0.291 ± 0.015
14.560 ± 0.741
1.8 ± 0.1
P = 0.000
B
0.139 ± 0.017
6.941 ± 0.871
1.6 ± 0.0
C
0.190 ± 0.015
9.486 ± 0.732
1.7 ± 0.1
1000 ()
A
0.406 ± 0.009
20.280 ± 0.457
1.7 ± 0.0
P = 0.020
B
0.242 ± 0.052
12.115 ± 2.585
1.8 ± 0.1
C
0.307 ± 0.052
15.347 ± 2.623
1.6 ± 0.1
A: ammonium sulfate DNA extraction method; B: Pinpoint Slide DNA Isolation System; C: TaKaRa DEXPAT; PTC: papillary thyroid carcinoma. Analysis of variance. Mean value ± standard deviation.
