Sperm Impairment by Sperm Agglutinating Factor Isolated from Escherichia coli: Receptor Specific Interactions
Figure 3
Representative cytogram for detection of apoptotic and necrotic sperm when exposed to the following for 0 or 30 min. (a) Spermatozoa alone, (b) SAF (150 μg) for 0 min, (c) SAF (150 μg) for 30 min, (d) SAF + receptor (125 μg) for 0 min, (e) SAF + receptor (125 μg) for 30 min, (f) SAF + receptor (150 μg) for 0 min, (g) SAF + receptor (150 μg) for 30 min. Sample cytogram stained using Annexin V/PI staining showing necrotic cells in Quadrant Q1, late apoptotic in Q2, live cells in Q3 and early apoptotic in Q4.
