548497.fig.003a
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548497.fig.003b
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548497.fig.003c
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548497.fig.003d
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548497.fig.003e
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Figure 3: Representative cytogram for detection of apoptotic and necrotic sperm when exposed to the following for 0 or 30 min. (a) Spermatozoa alone, (b) SAF (150 μg) for 0 min, (c) SAF (150 μg) for 30 min, (d) SAF  +  receptor (125 μg) for 0 min, (e) SAF + receptor (125 μg) for 30 min, (f) SAF + receptor (150 μg) for 0 min, (g) SAF + receptor (150 μg) for 30 min. Sample cytogram stained using Annexin V/PI staining showing necrotic cells in Quadrant Q1, late apoptotic in Q2, live cells in Q3 and early apoptotic in Q4.