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BioMed Research International
Volume 2013 (2013), Article ID 549037, 8 pages
http://dx.doi.org/10.1155/2013/549037
Research Article

Stability and Accuracy Assessment of Identification of Traditional Chinese Materia Medica Using DNA Barcoding: A Case Study on Flos Lonicerae Japonicae

1Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151 Malianwa North Road, Haidian District, Beijing 100193, China
2Agricultural College, Henan University of Science and Technology, Luoyang, Henan 471003, China
3Jiangsu Kanion Pharmaceutical Co. LTD, Lianyungang, Jiangsu 222001, China
4State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China
5College of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
6Beijing University of Chinese Medicine, Beijing 100102, China

Received 28 February 2013; Revised 8 May 2013; Accepted 22 May 2013

Academic Editor: H. M. Häggman

Copyright © 2013 Dianyun Hou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Flos Lonicerae Japonicae is a traditional Chinese materia medica which has an important function in the treatment of H1N1 influenza, hand-foot-and-mouth disease (HFMD), and severe acute respiratory syndromes (SARS). However, Flos Lonicerae Japonicae and its closely related species have been misused. Therefore, it is imperative to identify them accurately. DNA barcoding is a novel molecular identification method that provides a turning point in solving the difficulty in identifying traditional Chinese materia medica using traditional identification techniques. However, the stability and accuracy of DNA barcoding need further study. The present study aims to examine the stability and accuracy of the ITS2 and psbA-trnH regions in the identification of Flos Lonicerae Japonicae and its closely related species. The results showed that both regions can stably and accurately distinguish Flos Lonicerae Japonicae and its closely related species.

  1. Supplementary Material