Identification and Biotechnological Application of Novel Regulatory Genes Involved in Streptomyces Polyketide Overproduction through Reverse Engineering Strategy

Figure 3

(A) Authentic TMC standard. (B) TMC volumetric productivities measured by quantitative HPLC analyses of the ethyl acetate-extracted culture broths were 1.69 mg⋅L⁻¹ for the wild-type strain CK4412 (a), 5.44 mg⋅L⁻¹ for the wblA-tmc disruptant (b), 4.04 mg⋅L⁻¹ for CK4412-001/wblA-tmc (c), and 1.41 mg⋅L⁻¹ for CK4412/wblA-tmc (d). (C) Time-dependent tautomycetin volumetric productivities of Streptomyces. sp. CK4412WT (♦) S. sp. CK4412/ΔwblA _tmc (■) [36].