Table 1: Peptides identified by MALDI-TOF-MS analysis of the three TvLEGU-1 protein spots of Trichomonas vaginalis detected by the anti-TvLEGU-1r antibody.

Peptide numberaPositionb (aa)Number in the sequencec (av)dSpot 1eSpot 2eSpot 3eAmino acid sequencef

113–27I1749.9186++FAVLIAGSNDFYNYR
228–41II1734.9719+++HQADIFNMYQQLVK
341–66III2774.0025++GFDDQHITMMAYDDIALSSENPFR
442–66IV2930.1882++RGFDDQHITMMAYDDIALSSENPFR
575–84V1101.2126++HVNIYPGSSK
685–105VI2399.6528++INYAHNSVTADQFYTVLTTLK
7144–151VII911.4059++AFDTMEAK
8157–174VIII1994.1426++LFFGIEACYSGSVAAVFR
9157–176IX2193.5226+++LFFGIEACYSGSVAAVFRAK
10232–248X1931.1107++AQTTGSHVCYYGDVNMK

aConsecutive number assigned to the identified peptides. bPosition in amino acids (aa) residues of the identified peptides (start-end) in the aa sequence of the T. vaginalis TvLEGU-1 [38]. cArbitrary nomenclature used to describe the ten identified peptides in T. vaginalis TvLEGU-1 (see Supplementary Figure  1S in Supplementary Material available online at doi:10.1155/2012/561979). dPeptide mass average (av) identified by MALDI-TOF-MS after tryptic digestion of the three protein spots obtained from 2DE of protease-rich extracts from T. vaginalis grown in normal iron conditions (Figure 1). ePresence (+) or absence (−) of the peptides identified by MALDI-TOF-MS in the three TvLEGU-1 protein spots analyzed. fAmino acid sequence of the peptides obtained from a theoretical tryptic digestion of the deduced aa sequence of TvLEGU-1 [38] with identical masses to the experimental one (Supplementary Figure  1S).