The TvLEGU-1, a Legumain-Like Cysteine Proteinase, Plays a Key Role in Trichomonas vaginalis Cytoadherence

Table 3

The average number of T. vaginalis parasites attached to HeLa cell monolayers per coverslip with or without treatment using different proteinase inhibitors (Figure 8(B)).

Treatment	Parasites attached	Inhibition (%)
None^a	1450	0.0±13.9176
MA (5 𝜇M)^b	742	46.33±7.7764
MA (10 𝜇M)	556	60.78±5.6311
MA (50 𝜇M)	301	77.23±5.2907
TLCK (1 mM)	622	57.05±6.6416
Leupeptin (0.2 mM)	898	36.79±6.1359
E-64 (0.18 mM)	662	50.16±8.1047

^aNone corresponds to the control parasites without treatment with CP inhibitors. The number of parasites attached to the HeLa cell monolayer was taken as 100% adherence for comparative purpose. ^bMA corresponds to the Aza-Peptidyl Michael Acceptor, a legumain-specific inhibitor. These differences were statistically significant with a P < 0.001 (Figure 8(B)).