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BioMed Research International
Volume 2013 (2013), Article ID 563756, 7 pages
http://dx.doi.org/10.1155/2013/563756
Research Article

Monounsaturated Fatty Acids Are Substrates for Aldehyde Generation in Tellurite-Exposed Escherichia coli

1Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago de Chile, Chile
2Laboratorio de Microbiología y Bionanotecnología, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago de Chile, Chile
3Laboratorio de Bionanotecnología y Microbiología, Centro de Bioinformática y Biología Integrativa (CBIB), Facultad de Ciencias Biológicas, Universidad Andres Bello, Santiago de Chile, Chile

Received 30 April 2013; Accepted 9 July 2013

Academic Editor: Kota V. Ramana

Copyright © 2013 Gonzalo A. Pradenas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Reactive oxygen species (ROS) damage macromolecules and cellular components in nearly all kinds of cells and often generate toxic intracellular byproducts. In this work, aldehyde generation derived from the Escherichia coli membrane oxidation as well as membrane fatty acid profiles, protein oxidation, and bacterial resistance to oxidative stress elicitors was evaluated. Studies included wild-type cells as well as cells exhibiting a modulated monounsaturated fatty acid (MUFA) ratio. The hydroxyaldehyde 4-hydroxy 2-nonenal was found to be most likely produced by E. coli, whose levels are dependent upon exposure to oxidative stress elicitors. Aldehyde amounts and markers of oxidative damage decreased upon exposure to E. coli containing low MUFA ratios, which was paralleled by a concomitant increase in resistance to ROS-generating compounds. MUFAs ratio, lipid peroxidation, and aldehyde generation were found to be directly related; that is, the lower the MUFAs ratio, the lower the peroxide and aldehyde generation levels. These results provide additional evidence about MUFAs being targets for membrane lipid oxidation and their relevance in aldehyde generation.