Research Article

Suitability of Cell-Based Label-Free Detection for Cytotoxicity Screening of Carbon Nanotubes

Figure 1

Raw data of xCELLigence and Cell-IQ analysis software. (a) Growth curve of EAhy cells, untreated (green and red) and in the presence of 25 μg/mL MCNT20 (blue and pink). Image analysis by Cell-IQ software. (b) Classification of cells into stable (G1 cells, red), dividing cells (light green), necrotic cells (turquoise), and CNTs (dark green). Binucleated cells have to be identified manually (arrow head). (c) Time-related changes in the number of stable cells (red), dividing cells (light green), necrotic cells (turquoise), and CNTs (dark green) based on this classification are displayed in a graph.
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