Ropivacaine-Induced Contraction Is Attenuated by Both Endothelial Nitric Oxide and Voltage-Dependent Potassium Channels in Isolated Rat Aortae

Figure 3

The effect of 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) (a), methylene blue (b), fluconazole (b), and indomethacin (c) on ropivacaine concentration-response curves in endothelium-intact aortae. Data are shown as the mean ± SD and expressed as a percentage of the maximal contraction induced by isotonic 60 mM KCl. N indicates the number of rats from which descending thoracic aortic rings were derived. (a) 100% = 2.40±0.48 g (𝑛=6), 100% = 2.55±0.55 g (𝑛=6), and 100% = 2.70±0.61 g (𝑛=6) for untreated endothelium-intact aortae, endothelium-intact aortae treated with 1×10⁻⁶ M ODQ, and endothelium-intact aortae treated with 1×10⁻⁵ M ODQ, respectively. (b) 100% = 2.00±0.32 g (𝑛=9), 100% = 2.12±0.40 g (𝑛=7), and 100% = 2.10±0.42 g (𝑛=6) for untreated endothelium-intact aortae, endothelium-intact aortae treated with 1×10⁻⁶ methylene blue, and endothelium-intact aortae treated with 1×10⁻⁵ M fluconazole, respectively. (c) 100% = 2.08±0.27 g (𝑛=6), 100% = 2.09±0.40 g (𝑛=6), and 100% = 2.47±0.67 g (𝑛=6) for untreated endothelium-intact aortae, endothelium-intact aortae treated with 1×10⁻⁵ M indomethacin, and endothelium-intact aortae treated with 3×10⁻⁵ M indomethacin, respectively. (a) and (b): ^∗𝑃<0.001 versus control. (c): ^∗𝑃<0.001, ^†𝑃<0.05, and ^#𝑃<0.01 versus control.

(a)

(b)

(c)