Ropivacaine-Induced Contraction Is Attenuated by Both Endothelial Nitric Oxide and Voltage-Dependent Potassium Channels in Isolated Rat Aortae
Figure 7
Effect of ropivacaine on the activation of endothelial nitric oxide synthase (eNOS; ) by phosphorylation at Ser1777 in human umbilical vein endothelial cells (HUVECs). HUVECs were treated with ropivacaine ( M) for 5, 10, 30, and 60 min. (a) Phosphorylation of eNOS was examined by western blotting, as described in Methods. (b) Band intensities at 5, 10, 30, and 60 min were assessed by scanning densitometry. Data are shown as the mean ± SD. N indicates the number of independent experiments. versus control. t-eNOS: total eNOS; P-eNOS: phosphorylated eNOS.
