Figure 3: Effect of chemotherapeutic agents on MC540 fluorescence intensity. Cells were incubated with control vehicle or each agent and then harvested at the time when the alive and susceptible population reached a maximum. MC540 was added and assayed as described in Section 2. Data were analyzed by one-way analysis of variance followed by a Dunnett’s post test comparing methotrexate (), actinomycin D (), paclitaxel (), and daunorubicin () to control (). The overall effect of treatment was significant () with all treatments differing individually from the control ().