Research Article

The Omega-3 Polyunsaturated Fatty Acid DHA Induces Simultaneous Apoptosis and Autophagy via Mitochondrial ROS-Mediated Akt-mTOR Signaling in Prostate Cancer Cells Expressing Mutant p53

Figure 4

Inhibition of Akt-mTOR signaling contributes to the ROS-mediated apoptosis and autophagy. (a) Inhibition of Akt-mTOR signaling induced by DHA. PC3 (left) and DU145 (right) cells were incubated with 0−50 μM DHA for 24 h, and then the expression levels of Akt-mTOR pathway molecules were detected by Western blot analysis. (b) Effect of NAC on the expression levels of Akt-mTOR pathway molecules in DHA-treated cells. PC3 (left) and DU145 (right) cells were exposed to 50 μM DHA and/or 5 mM NAC for 12 h, and the expression levels of Akt-mTOR pathway molecules were examined by Western blot analysis.
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