About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 574136, 9 pages
http://dx.doi.org/10.1155/2013/574136
Research Article

Chitosan Combined with Poly-L-arginine as Efficient, Safe, and Serum-Insensitive Vehicle with RNase Protection Ability for siRNA Delivery

Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand

Received 29 April 2013; Revised 5 June 2013; Accepted 5 June 2013

Academic Editor: Kamla Pathak

Copyright © 2013 Samarwadee Plianwong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. M. Elbashir, J. Harborth, W. Lendeckel, A. Yalcin, K. Weber, and T. Tuschl, “Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells,” Nature, vol. 411, no. 6836, pp. 494–498, 2001. View at Publisher · View at Google Scholar · View at Scopus
  2. Y.-K. Oh and T. G. Park, “siRNA delivery systems for cancer treatment,” Advanced Drug Delivery Reviews, vol. 61, no. 10, pp. 850–862, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. S. Barik, “Silence of the transcripts: RNA interference in medicine,” Journal of Molecular Medicine, vol. 83, no. 10, pp. 764–773, 2005. View at Publisher · View at Google Scholar · View at Scopus
  4. S. Zhang, Y. Zhao, D. Zhi, and S. Zhang, “Non-viral vectors for the mediation of RNAi,” Bioorganic Chemistry, vol. 40, no. 1, pp. 10–18, 2012. View at Publisher · View at Google Scholar · View at Scopus
  5. S. Akhtar and I. F. Benter, “Nonviral delivery of synthetic siRNAs in vivo,” The Journal of Clinical Investigation, vol. 117, no. 12, pp. 3623–3632, 2007. View at Publisher · View at Google Scholar · View at Scopus
  6. A. de Fougerolles, H.-P. Vornlocher, J. Maraganore, and J. Lieberman, “Interfering with disease: a progress report on siRNA-based therapeutics,” Nature Reviews Drug Discovery, vol. 6, no. 6, pp. 443–453, 2007. View at Publisher · View at Google Scholar · View at Scopus
  7. D. J. Gary, N. Puri, and Y.-Y. Won, “Polymer-based siRNA delivery: perspectives on the fundamental and phenomenological distinctions from polymer-based DNA delivery,” Journal of Controlled Release, vol. 121, no. 1-2, pp. 64–73, 2007. View at Publisher · View at Google Scholar · View at Scopus
  8. W. J. Kim and S. W. Kim, “Efficient siRNA delivery with non-viral polymeric vehicles,” Pharmaceutical Research, vol. 26, no. 3, pp. 657–666, 2009. View at Publisher · View at Google Scholar · View at Scopus
  9. A. C. Richards Grayson, A. M. Doody, and D. Putnam, “Biophysical and structural characterization of polyethylenimine-mediated siRNA delivery in vitro,” Pharmaceutical Research, vol. 23, no. 8, pp. 1868–1876, 2006. View at Publisher · View at Google Scholar · View at Scopus
  10. B. Urban-Klein, S. Werth, S. Abuharbeid, F. Czubayko, and A. Aigner, “RNAi-mediated gene-targeting through systemic application of polyethylenimine (PEI)-complexed siRNA in vivo,” Gene Therapy, vol. 12, no. 5, pp. 461–466, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. M. L. Patil, M. Zhang, S. Betigeri, O. Taratula, H. He, and T. Minko, “Surface-modified and internally cationic polyamidoamine dendrimers for efficient siRNA delivery,” Bioconjugate Chemistry, vol. 19, no. 7, pp. 1396–1403, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. J. Zhou, J. Wu, N. Hafdi, J.-P. Behr, P. Erbacher, and L. Peng, “PAMAM dendrimers for efficient siRNA delivery and potent gene silencing,” Chemical Communications, no. 22, pp. 2362–2364, 2006. View at Publisher · View at Google Scholar · View at Scopus
  13. S. Mao, W. Sun, and T. Kissel, “Chitosan-based formulations for delivery of DNA and siRNA,” Advanced Drug Delivery Reviews, vol. 62, no. 1, pp. 12–27, 2010. View at Publisher · View at Google Scholar · View at Scopus
  14. K. A. Howard, U. L. Rahbek, X. D. Liu et al., “RNA interference in vitro and in vivo using a chitosan/siRNA nanoparticle system,” Molecular Therapy, vol. 14, no. 4, pp. 476–484, 2006. View at Publisher · View at Google Scholar · View at Scopus
  15. H. Katas and H. O. Alpar, “Development and characterisation of chitosan nanoparticles for siRNA delivery,” Journal of Controlled Release, vol. 115, no. 2, pp. 216–225, 2006. View at Publisher · View at Google Scholar · View at Scopus
  16. X. Liu, K. A. Howard, M. Dong et al., “The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing,” Biomaterials, vol. 28, no. 6, pp. 1280–1288, 2007. View at Publisher · View at Google Scholar · View at Scopus
  17. K. A. Howard, S. R. Paludan, M. A. Behlke, F. Besenbacher, B. Deleuran, and J. Kjems, “Chitosan/siRNA nanoparticle-mediated TNF-α knockdown in peritoneal macrophages for anti-inflammatory treatment in a murine arthritis model,” Molecular Therapy, vol. 17, no. 1, pp. 162–168, 2009. View at Publisher · View at Google Scholar · View at Scopus
  18. T. Rojanarata, P. Opanasopit, S. Techaarpornkul, T. Ngawhirunpat, and U. Ruktanonchai, “Chitosan-thiamine pyrophosphate as a novel carrier for siRNA delivery,” Pharmaceutical Research, vol. 25, no. 12, pp. 2807–2814, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. S. Techaarpornkul, S. Wongkupasert, P. Opanasopit, A. Apirakaramwong, J. Nunthanid, and U. Ruktanonchai, “Chitosan-mediated siRNA delivery in vitro: effect of polymer molecular weight, concentration and salt forms,” AAPS PharmSciTech, vol. 11, no. 1, pp. 64–72, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. S. Xu, M. Dong, X. Liu, K. A. Howard, J. Kjems, and F. Besenbacher, “Direct force measurements between siRNA and chitosan molecules using force spectroscopy,” Biophysical Journal, vol. 93, no. 3, pp. 952–959, 2007. View at Publisher · View at Google Scholar · View at Scopus
  21. T. Sato, T. Ishii, and Y. Okahata, “In vitro gene delivery mediated by chitosan. Effect of pH, serum, and molecular mass of chitosan on the transfection efficiency,” Biomaterials, vol. 22, no. 15, pp. 2075–2080, 2001. View at Publisher · View at Google Scholar · View at Scopus
  22. M. Lavertu, S. Méthot, N. Tran-Khanh, and M. D. Buschmann, “High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation,” Biomaterials, vol. 27, no. 27, pp. 4815–4824, 2006. View at Publisher · View at Google Scholar · View at Scopus
  23. M. E. Martin and K. G. Rice, “Peptide-guided gene delivery,” The AAPS Journal, vol. 9, no. 1, pp. E18–E29, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. P. Saccardo, A. Villaverde, and N. González-Montalbán, “Peptide-mediated DNA condensation for non-viral gene therapy,” Biotechnology Advances, vol. 27, no. 4, pp. 432–438, 2009. View at Publisher · View at Google Scholar · View at Scopus
  25. M. Yamagata, T. Kawano, K. Shiba, T. Mori, Y. Katayama, and T. Niidome, “Structural advantage of dendritic poly(l-lysine) for gene delivery into cells,” Bioorganic & Medicinal Chemistry, vol. 15, no. 1, pp. 526–532, 2007. View at Publisher · View at Google Scholar · View at Scopus
  26. S. Shahana, C. Kampf, and G. M. Roomans, “Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro,” Mediators of Inflammation, vol. 11, no. 3, pp. 141–148, 2002. View at Publisher · View at Google Scholar · View at Scopus
  27. E.-J. Kim, G. Shim, K. Kim, I. C. Kwon, Y.-K. Oh, and C.-K. Shim, “Hyaluronic acid complexed to biodegradable poly L-arginine for targeted delivery of siRNAs,” The Journal of Gene Medicine, vol. 11, no. 9, pp. 791–803, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. S. M. Noh, M. O. Park, G. Shim et al., “Pegylated poly-l-arginine derivatives of chitosan for effective delivery of siRNA,” Journal of Controlled Release, vol. 145, no. 2, pp. 159–164, 2010. View at Publisher · View at Google Scholar · View at Scopus
  29. P. Opanasopit, J. Tragulpakseerojn, A. Apirakaramwong, T. Ngawhirunpat, and T. Rojanarata, “Chitosan enhances transfection efficiency of cationic polypeptides/DNA complexes,” International Journal of Pharmaceutics, vol. 410, no. 1-2, pp. 161–168, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. J. Kim, S. H. Lee, J. Choe, and T. G. Park, “Intracellular small interfering RNA delivery using genetically engineered double-stranded RNA binding protein domain,” The Journal of Gene Medicine, vol. 11, no. 9, pp. 804–812, 2009. View at Publisher · View at Google Scholar · View at Scopus