Table 1: Composition of HFA 227 formulations maintaining constant fluorescein Na and ethanol concentration ( ).

FormulationPluronic L81 (% w/w)Ethanol (% w/w)Fluorescein Na (% w/w)

1.22% w/w Pluronic L811.2213.30.04
5.45% w/w Pluronic L815.4513.40.04
0% w/w Pluronic L81 13.30.04

Formulation 0% w/w Pluronic L81 contained 1.22% w/w of deionized water as a replacement for Pluronic L81 to maintain mass balance.