Cytokeratin 18 Is Not Required for Morphogenesis of Developing Prostates but Contributes to Adult Prostate Regeneration
Figure 5
CK18 is required for DLP branching in prostate regeneration. (a) Prostates are carefully dissected after castration and androgen replacement. The gross anatomic appearance reveals no obvious difference between WT and CK18−/− prostates. Scale bars are 2000 μm. (b, c) The images of microdissected prostates from WT (a) and CK18−/− (b) mice after castration and androgen replacement show compromised branching pattern in DLP but not in VP and AP of CK18−/− prostate. Four mice of each group are used in the experiment. Only representative pictures are shown. The scale bar in AP image is 2000 μm; scale bars in VP and DLP images are 1000 μm. (d, e) Bar graphs show significantly reduced umbers of ductal tips (mean ± SEM) and branch points in CK18−/− DLP (mean ± SEM, **denotes ).
