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BioMed Research International
Volume 2013 (2013), Article ID 578276, 6 pages
Research Article

Persistence or Clearance of Human Papillomavirus Infections in Women in Ouro Preto, Brazil

1Laboratório de Genética, Instituto Butantan, Avenida Vital Brasil 1500, 05503-900 São Paulo, SP, Brazil
2Departamento de Análises Clínicas Escola de Farmácia, Universidade de Ouro Preto (UFOP), Rua Costa Sena 171, 35400-000 Ouro Preto, MG, Brazil
3Laboratório de Tocoginecologia, Departamento de Ginecologia, Universidade Federal de São Paulo, 04039-032 São Paulo, SP, Brazil
4Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), 50670-901 Recife, PE, Brazil
5Programa de Pós-Graduação Interunidades em Biotecnologia, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil
6Departamento de Biologia, Universidade Federal da Integração Latino-Americana (UNILA), 85867-970 Foz do Iguaçú, PR, Brazil

Received 2 April 2013; Accepted 6 September 2013

Academic Editor: Marcelo Palma Sircili

Copyright © 2013 P. M. Miranda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Persistent high-risk (HR) human papillomavirus (HPV) infection is necessary for development of precursor lesions and cervical cancer. We investigate persistence and clearance of HPV infections and cofactors in unvaccinated women. Cervical samples of 569 women (18–75 years), received for routine evaluation in the Health Department of Ouro Preto, Brazil, were collected and subjected to PCR (MY09/11 or GP5+/6+ primers), followed by RFLP or sequencing. All women were interviewed to collect sociodemographic and behavioral information. Viral infection persistence or clearance was reevaluated after 24 months and was observed in 59.6% and 40.4% of women, respectively. HPVs 16, 33, 59, 66, 69, and 83 (HR) were the most persistent types whereas HPVs 31, 45, and 58 were less persistent. Clearance or persistence did not differ between groups infected by HPVs 18, 53, and 67. In low-risk (LR) types, HPV 6 infected samples were associated with clearance, while HPV 11, 61, 72, or 81 infected samples were persistent in the follow-up. No statistically significant association was detected between persistent HPV infections and sociodemographic and behavioral characteristics analyzed. To study persistence or clearance in HPV infection allows the identification of risk groups, cofactors, and strategies for prevention of cervical cancer.