[Retracted] The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin Fibroblasts In Vitro
Figure 3
The effects of different concentrations or the neutralizing antibodies of cytokines in the 50% ASC-CM or DMEM on skin fibroblasts migration utilizing transwell assay. Migration was observed to be inhibited by EGF at 20 ng/mL, while being obviously promoted at 100 ng/mL (a). Fibroblasts migration was significantly promoted by PDGF-AA at 50 ng/mL (the optimal concentration) and 100 ng/mL, and the promoting effect was obviously inhibited when neutralizing antibody of PDGF-AA was added (b). VEGF was observed to make contribution to fibroblasts migration, and the optimal concentration was 20 ng/mL (), while this contribution was restrained by its neutralizing antibody () (c). The bFGF at 20 ng/mL (the optimal concentration), 50 ng/mL, and 100 ng/mL obviously promoted fibroblast migration (), and its neutralizing antibody obviously inhibited fibroblast migration () (d). In addition, compared with the control group (not containing ASC-CM) and each single cytokine group, specially the EGF, 50% ASC-CM significantly promoted the fibroblasts migration. *(); **(); NA: neutralizing antibody group. Each group was compared with 50% ASC-CM without any additional cytokines.
