[Retracted] The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin Fibroblasts In Vitro

Figure 5

The effects of EGF, VEGF, PDGF, and bFGF in 50% ASC-CM on skin fibroblasts migration utilizing scrape-wound healing assay, and the optimal concentration of cytokines (reference to the result of transwell assay) in 50% ASC-CM was chosen to further confirm its effect. Results showed that fibroblasts migration was exactly inhibited by 20 ng/mL of EGF (b, g), while it was promoted by 20 ng/mL VEGF (c, h), 50 ng/mL PDGF (d, i), and 20 ng/mL bFGF (e, j). Their effects could be restrained by their neutralizing antibody (k–n). This result was consistent with the transwell assay.

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