Table 4: Trypsin inhibitory, lectin, urease, and toxic activities present in the crude extract from B. cheilantha seeds and their stability to heat treatment.

ActivitiesHeat treatment*
0 min30 min60 min90 min

Trypsin inhibitory**31.5 ± 1.9a20.7 ± 0.1b11.4 ± 0.4c1.2 ± 0.1d
Lectin793.6 ± 1.2NDNDND
Urease#32.9 ± 1.3a19.7 ± 0.3b7.4 ± 0.5c5.2 ± 0.5d
Toxic***NLNLNLNL

All values are means ± standard deviation of triplicates.
* B. cheilantha seed meal was submitted to boiling at 92°C at different periods of time.
**Trypsin inhibitory activity is expressed as mg of trypsin inhibited per g of meal.
Lectin activity is expressed as haemagglutinating units per g of meal (UH/gF). Haemagglutinating unit is the reciprocal of the highest dilution giving a visible agglutination.
ND: not detected.
#Urease activity is shown as units of enzyme per g of meal. The units were calculated from Sigma information that 1 g of pure enzyme contains 870.000 units.
***Toxic activity is represented as LD50, 50% lethal dose. One LD50 designates the amount of protein in g/Kg of mouse body weight producing convulsion and death of 50% of tested animals injected by intraperitoneal route.
NL: not lethal even at a dose of 1.0 g per Kg of mouse body weight.