Chemical Composition, Nutritive Value, and Toxicological Evaluation of Bauhinia cheilantha Seeds: A Legume from Semiarid Regions Widely Used in Folk Medicine

Table 4

Trypsin inhibitory, lectin, urease, and toxic activities present in the crude extract from B. cheilantha seeds and their stability to heat treatment.

Activities	Heat treatment*
	0 min	30 min	60 min	90 min
Trypsin inhibitory**	31.5 ± 1.9^a	20.7 ± 0.1^b	11.4 ± 0.4^c	1.2 ± 0.1^d
Lectin^∧	793.6 ± 1.2	ND^†	ND	ND
Urease^#	32.9 ± 1.3^a	19.7 ± 0.3^b	7.4 ± 0.5^c	5.2 ± 0.5^d
Toxic***	NL^‡	NL	NL	NL

All values are means ± standard deviation of triplicates.
* B. cheilantha seed meal was submitted to boiling at 92°C at different periods of time.
**Trypsin inhibitory activity is expressed as mg of trypsin inhibited per g of meal.
^∧Lectin activity is expressed as haemagglutinating units per g of meal (UH/gF). Haemagglutinating unit is the reciprocal of the highest dilution giving a visible agglutination.
^†ND: not detected.
^#Urease activity is shown as units of enzyme per g of meal. The units were calculated from Sigma information that 1 g of pure enzyme contains 870.000 units.
***Toxic activity is represented as LD₅₀, 50% lethal dose. One LD₅₀ designates the amount of protein in g/Kg of mouse body weight producing convulsion and death of 50% of tested animals injected by intraperitoneal route.
^‡NL: not lethal even at a dose of 1.0 g per Kg of mouse body weight.