Expression Systems and Species Used for Transgenic Animal Bioreactors

Table 6

Recombinant proteins produced from transgenic rabbits.

Expressed proteins	Promoter	Expressed protein	References
Human α1-antitrypsin	Human α 1-antitrypsin DNA	1 g/L in plasma	[17]
Human α-glucosidase	Bovine αs1-casein	8 g/L	[37]
	N-acetyl-β-glucosaminyl	NA	[38]
Human C1 inhibitor	NA	NA	[39]
Human clotting factor VIII	Mouse WAP	NA	[40]
	Mouse WAP	0.005–0.161 g/L	[41]
Human erythropoietin	Rabbit WAP	0.0000003 g/L	[42]
	Rabbit WAP	NA	[43]
	Bovine β-lactoglobulin	0.5 g/L	[44]
	Rabbit WAP	NA	[45]
	Rabbit WAP	60–178 IU/L	[46]
Human extracellular SOD	Mouse WAP	3 g/L	[47]
Human growth hormone	Mouse WAP	0.000012 g/L	[48]
	Rat WAP	0.5–1.0 g/L	[49]
	Rat WAP	0.010 g/L	[50]
Human IL-2	Rabbit β-casein	0.0005 g/L	[51]
Human insulin-like growth factor	Bovine αs1-casein	1 g/L	[52]
	Bovine αs1-casein	0.3 g/L	[53]
	Bovine αs1-casein	0.678 g/L	[54]
Human nerve growth factor β	Bovine αs1-casein	0.25 g/L	[55]
	Adenoviral	0.346 g/L	[56]
Human tPA	Bovine αs1-casein	0.00005 g/L	[57]
Bovine chymosin	Bovine αs1-casein	1.5 g/L	[58]
Bovine FSH	Bovine αs1-casein	0.1 g/L	[59]
Equine chorionic gonadotropin	Rabbit WAP	0.022 g/L	[60]
Salmon calcitonin	Ovine β-lactoglobulin	2.1 g/L	[61]
Human protein C	Mouse WAP	0.0000001–0.0000003 g/L	[62]
TNAP	Human WAP	NA	[63]
Human lactoferrin	Adenoviral	2.3 g/L	[64]
Human interferon beta	NA	2.2–7.2 × 107 IU/L	[65]
Human antithrombin	Adenoviral	4.8 g/L	[66]

FSH: follicle stimulating hormone; IL-2: interleukin-2; NA: not available; SOD: superoxide dismutase; TNAP: tissue-nonspecific alkaline phosphatase; tPA: tissue plasminogen activator; WAP: whey acidic protein.